Engineering of human-type O-glycosylation inNicotiana benthamianaplants
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چکیده
منابع مشابه
Engineering of human-type O-glycosylation in Nicotiana benthamiana plants
Therapeutic properties of recombinant proteins are very often affected by the composition and heterogeneity of their glycans. Conventional expression systems for recombinant pharmaceutical proteins typically do not address this problem and produce a mixture of glycoforms that are neither identical to human glycans nor optimized for enhanced efficacy. In terms of glycosylation, plants offer cert...
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Glycosylation is the most abundant and complex posttranslational modification to be considered for recombinant production of therapeutic proteins. Mucin-type (N-acetylgalactosamine [GalNAc]-type) O-glycosylation is found in eumetazoan cells but absent in plants and yeast, making these cell types an obvious choice for de novo engineering of this O-glycosylation pathway. We previously showed that...
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Mucin-type O-glycosylation plays a crucial role in several physiological and pathological processes of the gastric tissue. Modifications in enzymes responsible for key glycosylation steps and the consequent abnormal biosynthesis and expression of their glycan products constitute well-established molecular hallmarks of disease state. This review addresses the major role played by mucins and asso...
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O-Glycosylation is one of the most important posttranslational modifications of proteins. It takes part in protein conformation, protein sorting, developmental processes and the modulation of enzymatic activities. In vertebrates, the basics of the biosynthetic pathway of O-glycans are already well understood. However, the regulation of the processes and the molecular aspects of defects, especia...
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Department of Molecular Biology and Genetics, Faculty of Science and Technology, Aarhus University, Flakkebjerg, 4200 Slagelse, Denmark (Z.Y.); Department of Plant Biology and Biotechnology, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C, Denmark (D.P.D., P.U., B.L.P.); Department of Agriculture and Ecology, Faculty of Science, University of Copenhagen, 1871 Frederiksberg C,...
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ژورنال
عنوان ژورنال: Bioengineered
سال: 2013
ISSN: 2165-5979,2165-5987
DOI: 10.4161/bioe.22857